Endometrial dating histology siblingsordating com
We evaluated the ability of the endometrial biopsy to detect luteal adequacy in a small population of fertile, midreproductive-aged women.We collected daily hormonal information on the women to allow us to determine a relationship between hormones and histology, should it exist.Histological evaluation of the endometrial biopsies was carried out by three authors (N. There was agreement among all three observers to within 2 days in most cases.When disparity was observed between glands and stroma, each component was dated separately, and the overall histological date was taken as the mean.The remaining two thirds were divided equally, placed into nalgene tubes, and snap-frozen in liquid nitrogen for biochemical and genetic studies (not reported herein).
Hormonal analyses of E1c and Pdg were carried out using previously described methods (10, 11).
Thus, studies that clearly delineate which histologic parameters serve as the greatest source of disagreement for pathologists provide a valuable framework for further refinement of the criteria for endometrial dating.
Meanwhile, continued use of the criteria of Noyes et al for endometrial dating is recommended until more precise modalities for assessing the adequacy of endometrial maturation are available.
Detection of luteal inadequacy has been traditionally performed by late luteal endometrial biopsy (4, 5).
Dating the endometrium histologically is customarily accomplished using standards set by Noyes 6–8 days after ovulation) may provide greater detection of histological abnormalities (8) and, hence, greater sensitivity of the clinical test.